Guilluy Lab @ NCSU

Guilluy Lab

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Nuclear Dynamics in Physiology and Diseases

Nuclear Dynamics in Physiology and Diseases Nuclear Dynamics in Physiology and Diseases Nuclear Dynamics in Physiology and Diseases

Our lab combines interdisciplinary approaches to investigate how nuclear structure and dynamics control cellular processes.

Nuclear Dynamics in Physiology and Diseases

Nuclear Dynamics in Physiology and Diseases Nuclear Dynamics in Physiology and Diseases Nuclear Dynamics in Physiology and Diseases

Our lab combines interdisciplinary approaches to investigate how nuclear structure and dynamics control cellular processes.

About

The nuclear envelope and the control of cell growth

The nuclear envelope and the control of cell growth

The nuclear envelope and the control of cell growth

  

How cells adjust to the spatial and mechanical properties of their environment is a central mechanism in cell biology, under physiological conditions and diseases. Our research aims to identify nuclear mechanisms that control cell growth in response to mechanical constraints of the cellular micro-environment.

Nuclear signaling in response to compression

The nuclear envelope and the control of cell growth

The nuclear envelope and the control of cell growth

Cells in tissue can be exposed to compressive forces which  trigger signaling pathway that eventually regulate gene expression and cytoskeletal remodeling. We aim to identify the nuclear signaling molecules that mediate this response. 

Chromatin Proteome

The nuclear envelope and the control of cell growth

Chromatin Proteome

 Epithelial cells can be exposed ; however, despite these stressors, they maintain functional stability and display remarkable resilience. We aim to understand how these cells preserve nuclear structure and function under such conditions.

Research Projects### Under Construction#####

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PUBLICATIONS

Publications

  

  • Brun-Cosme-Bruny M., Pernet L., Elias K., Guilluy C., Oddou C. and Dolega M. Calcium waves and nuclear tension changes coordinate mechanical stress dissipation in locally folded epithelia. iScience. 2026. doi: 10.1016/j.isci.2025.114477
  • Monaghan-Benson E., Aureille J. and Guilluy C., ECM stiffness regulates lung fibroblast survival through RasGRF1 dependent signaling. Journal of Biological Chemistry. 2025. doi.org/10.1016/j.jbc.2025.108161 
  • Belaadi N and Guilluy C. Life outside the LINC: DO SUN proteins have LINC independent functions? BioEssays. 2024 Aug;46(8):e2400034. doi: 10.1002/bies.202400034. Epub 2024 May 27
  • Buglak D., Bougaran P., Kulikauskas, Ziqing Liu Z., Monaghan-Benson E., Gold A., Marvin A., Burciu A., Tanke N., Ricketts S., Kinghorn K, Oatley M., Johnson B, Shiau C., Rohers S., Guilluy C. and Bautch V Nuclear SUN1 stabilizes endothelial cell junctions via microtubules to regulate blood vessel formation. eLife. 2023 Mar 29;12:e83652. DOI: 10.7554/eLife.83652
  • Belaadi N, Pernet L, Aureille J, Chadeuf G, Rio M, Vaillant N, Vitiello E, Lafanechère L, Loirand G and Guilluy C. SUN2 regulates mitotic duration in response to extracellular matrix rigidity. PNAS 2022 Nov 8;119(45):e2116167119. DOI: 10.1073/pnas.2116167119 
  • Blonski S., Aureille J., Badawi S., Zaremba D., Pernet L., Grichine A., Fraboulet F., Korczyk P.M., Recho P., Guilluy C., and Dolega M.E. Direction of epithelial folding defines impact of mechanical forces on epithelial state. Dev. Cell. 2021. Volume 56, Issue 23, 6 December 2021, Pages 3222-3234.e6. 
  • Aureille J., Pezet M., Pernet L., Mazzega J., Grichine A., Guilluy C. and M. E. Dolega. Cell fluorescence photo-activation as a method to select and study cellular sub populations grown in mechanically heterogeneous environments. Molecular Biology of the Cell Vol. 32, No. 16 2021. 
  • Guilluy C. and Dolega M. Syndecan-4 forces integrins to cooperate. Nature materials. 020 Jun;19(6):587-588. DOI: 10.1038/s41563-020-0689-5 
  • Aureille J., Buffière-Ribot V., Harvey B.E., Boyault C., Pernet L., Andersen T., Bacola G., Balland M., Fraboulet S., Van Landeghem L. and Guilluy C. Nuclear envelope deformation controls cell cycle progression in response to mechanical force. EMBO Rep. 2019 Aug 1. 20 (9). DOI: 10.15252/embr.201948084 
  • Umana-Diaz C., Pichol-Thievend C., Marchand MF., Atlas Y., Salza R., Malbouyres M., Barret A., Teillon J., Ardidie-Robouant C., Ruggiero F., Monnot C., Girard P., Guilluy C., Ricard-Blum S., Germain S. and Muller L. Scavenger Receptor Cysteine-Rich Domains of Lysyl Oxidase-Like2 Regulate Endothelial ECM and Angiogenesis Through Non-Catalytic Scaffolding Mechanisms. Matrix Biol.     2019, 88, pp.33-52. DOI: 10.1016/j.matbio.2019.11.003
  • Belbachir N., Portero V., Al Sayed Z.R., Gourraud J.B., Dilasser F., Jesel L., Guo H., Wu H., Gaborit N., Guilluy C., Girardeau A., Bonnaud S., Simonet F., Karakachoff M., Pattier S., Scott C., Burel S., Marionneau C., Chariau C., Gaignerie A., David L., Genin E., Deleuze J.F., Dina C., Sauzeau V., Loirand G., Baró I., Schott J.J., Probst V., Wu J.C., Redon R., Charpentier F. and Le Scouarnec S. RRAD mutation causes electrical and cytoskeletal defects in cardiomyocytes derived from a familial case of Brugada syndrome. Eur  Heart J. 2019 May 21. 40 (37). DOI: 10.1093/eurheartj/ehz308
  • Belaadi N., Millon-Frémillon A., Aureille J. and Guilluy C. Analyzing      Mechanotransduction Through the LINC Complex in Isolated Nuclei. Methods Molecular Biology, Methods Mol Biol. 2018;1840:73-80. DOI: 10.1007/978-1-4939-8691-0_7 
  • Aureille J., Belaadi N. and Guilluy C. Mechanotransduction via the nuclear envelope: a distant reflection of the cell surface. Curr. Opin. Cell Biol. 2017 Feb; 44:59-67. DOI: 10.1016/j.ceb.2016.10.003 
  • Millon-Frémillon A., Aureille J. and Guilluy C. Analyzing Cell Surface Adhesion Remodeling in Response to Mechanical Tension Using Magnetic Beads. J Vis Exp. 2017 Mar 8;(121). DOI: 10.3791/55330 
  • Duchalais E., Guilluy C., Nedellec S., Touvron M., Bessard A., Touchefeu Y., Bossard C., Boudin H., Louarn G., Neunlist M. and Van Landeghem L. Colorectal Cancer Cells Adhere to and Migrate Along the Neurons of the Enteric Nervous System. Cell Mol Gastroenterol Hepatol. 2017 Oct 14;5(1):31-49. DOI: 10.1016/j.jcmgh.2017.10.002
  • Burridge K and Guilluy C. Focal adhesions, stress fibers and mechanical tension. Exp Cell Res. 2016 Apr 10;343(1):14-20. DOI: 10.1016/j.yexcr.2015.10.029 
  • Marjoram R.J., Guilluy C. and Burridge K. Using magnets and magnetic beads      to dissect signaling pathways activated by mechanical tension applied to cells. Methods. 2016 Feb 1; 94:19-26. DOI: 10.1016/j.ymeth.2015.09.025. Epub 2015 Sep 30. DOI: 10.1016/j.jcmgh.2017.10.002
  • Belaadi N., Aureille J. Guilluy C. Under Pressure: Mechanical Stress Management in the Nucleus. Cells. 2016 Jun 14;5(2). pii: E27. DOI: 10.3390/cells5020027 
  • Guilluy C. and Burridge K. Nuclear mechanotransduction: forcing the nucleus to respond. Nucleus. 2015;6(1):19-22. DOI: 10.1080/19491034.2014.1001705 
  • Collins C., Osborne L.D., Guilluy C., Chen Z., O'Brien E.T. 3rd, Reader J.S., Burridge K., Superfine R. and Tzima E. Haemodynamic and extracellular matrix cues regulate the mechanical phenotype and stiffness of aortic endothelial cells. Nat. Commun. 2014 Jun 11; 5:3984. DOI: 10.1038/ncomms4984
  • Bays J.L., Peng X., Tolbert C.E., Guilluy C., Angell A.E., Pan Y., Superfine R., Burridge K. and DeMali K.A. Vinculin phosphorylation differentially regulates mechanotransduction at cell-cell and cell-matrix adhesions. J. Cell Biol. 2014 Apr 28;205(2):251-63. DOI: 10.1083/jcb.201309092
  • Guilluy C., Osborne L.D., Van Landeghem L., Superfine R., Garcia-mata R. and Burridge K. Isolated nuclei adapt to force and reveal a mechanotransduction pathway within the nucleus. Nature Cell Biol. 2014 Mar 9. DOI: 10.1038/ncb2927 
  • Lessey-Morillon E.C., Osborne      L.D., Monaghan-Benson E., Guilluy C., O'Brien E.T., Superfine R. and      Burridge K. The RhoA guanine nucleotide exchange factor, LARG, mediates ICAM-1-dependent mechanotransduction in endothelial cells to stimulate transendothelial migration. J. Immunol. 2014      Apr 1;192(7):3390-8. DOI: 10.4049/jimmunol.1302525
  • Thompson W.R., Guilluy C., Xie      Z., Sen B., Brobst K.E., Yen S.S., Uzer G., Styner M., Case N., Burridge K. and Rubin J. Mechanically activated Fyn utilizes mTORC2 to regulate RhoA and adipogenesis in mesenchymal stem cells. Stem Cells. 2013      Nov;31(11):2528-37. DOI: 10.1002/stem.1476
  • Lessey E.C., Guilluy C. and Burridge K. From mechanical force to RhoA activation. Biochemistry. 2012 Sep 25;51(38):7420-32. DOI: 10.1021/bi300758e
  • Collins C., Guilluy C., Welch C.,      O'Brien E.T., Hahn K., Superfine R., Burridge K. and Tzima E. Localized tensional forces on PECAM-1 elicit a      global mechanotransduction response via the integrin-RhoA pathway. Curr. Biol. 2012 Nov 20;22(22):2087-94. DOI: 10.1016/j.cub.2012.08.051
  • Guilluy C., Dubash A. and Garcia-Mata R. Analysis of RhoA and Rho GEF activity in whole cells and the cell nucleus. Nature Protocols. 2011 Dec      1;6(12):2050-60. DOI: 10.1038/nprot.2011.411
  • Guilluy C., Garcia-Mata R. and Burridge K. Rho protein crosstalk: another social network? Trends Cell Biol. 2011 Dec;21(12):718-26. DOI: 10.1016/j.tcb.2011.08.002
  • Shen K., Tolbert C.E., Guilluy C.,      Swaminathan V.S., Berginski M.E., Burridge K., Superfine R., Campbell S.L. The Vinculin C-terminal Hairpin Mediates F-actin Bundle Formation, Focal Adhesion, and Cell Mechanical Properties. J. Biol. Chem. 2011 Dec 30;286(52):45103-15. DOI: 10.1074/jbc.M111.244293
  • Sen B., Guilluy C., Xie Z., Case N., Styner M., Thomas J., Oguz I., Rubin C., Burridge K. and Rubin J. Mechanically induced focal adhesion assembly amplifies anti-adipogenic pathways in mesenchymal stem cells. Stem Cells. 2011 Nov;29(11):1829-36. DOI: 10.1002/stem.732
  • Guilluy C., Zhang Z., Bhende P.M., Sharek L., Wang L., Burridge K. and Damania B. Latent KSHV infection increases the vascular permeability of human endothelial cells. Blood. 2011 Aug 31. 118 (19), pp.5344-5354. DOI: 10.1182/blood-2011-03-341552
  • Guilluy C., Swaminathan V., Garcia-Mata R., O'Brien E.T., Superfine R. and Burridge K. The Rho GEFs LARG and GEF-H1 regulate the mechanical response to force on integrins. Nature      Cell Biol. 2011 Jun;13(6):722-7. Epub 2011 May 15.      DOI: 10.1038/ncb2254
  • Gayard M., Guilluy C., Rousselle A., Viollet B., Henrion D., Pacaud P., Loirand G. and Rolli-Derkinderen M. AMPK Alpha 1-Induced RhoA Phosphorylation Mediates Vasoprotective Effect of Estradiol. Arterioscler Thromb Vasc Biol. 2011 Aug 18. 31      (11) ,pp.2634-U704. DOI: 10.1161/ATVBAHA.111.228304
  • Dubash A.D., Guilluy C., Srougi M.C., Boulter E., Burridge K. and García-Mata R. The small GTPase RhoA localizes to the nucleus and is activated by Net1 and DNA damage signals. PLoS One. 2011 Feb 24;6(2): e17380. DOI: 10.1371/journal.pone.0017380
  • Duong-Quy S., Dao P., Hua-Huy T., Guilluy C., Pacaud P. and Dinh-Xuan A.T. Increased Rho-kinase expression and activity and pulmonary endothelial dysfunction in smokers with normal lung function. Eur. Respir. J. 2011 Feb;37(2):349-55. Epub 2010 Jun 7. DOI: 10.1183/09031936.00056610
  • Boulter E., Garcia-Mata R., Guilluy C., Dubash A., Rossi G., Brennwald P.J. and Burridge K. Regulation of Rho GTPase crosstalk, degradation and activity by RhoGDI1. Nature Cell Biol. 2010 May;12(5):477-83. Epub 2010 Apr 18. DOI:      10.1038/ncb2049
  • Guilluy C., Brégeon J., Toumaniantz G., Rolli-Derkinderen M., Retailleau K., Loufrani L., Henrion D., Scalbert E., Bril A., Torres R.M., Offermanns S., Pacaud P. and Loirand G. The Rho exchange factor Arhgef1 mediates the effects of angiotensin II on vascular tone and blood      pressure. Nature Med. 2010 Feb;16(2):183-90. Epub 2010 Jan      24. DOI: 10.1038/nm.2079
  • Guilluy C., Eddahibi S., Agard      C., Savale L., Humbert M., Fadel E., Adnot S., Pacaud P. and Loirand G.      RhoA and Rho kinase activation in human pulmonary hypertension - Role of      5-HT signaling Am. J. Respir. Crit. Care Med. 2009      Jun 15;179(12):1151-8. DOI: 10.1164/rccm.200805-691OC
  • Guilluy C., Rolli-Derkinderen M.,      Loufrani L., Bourge A., Sabourin L., Henrion D., Loirand G. and Pacaud P.      Ste20-related kinase SLK phosphorylates Ser 188 of RhoA to induce      vasodilation in response to Angiotensin II type 2 receptor activation. Circ      Res. 2008 Apr 17. DOI: 10.1161/CIRCRESAHA.107.164764
  • Guilluy C., Rolli-Derkinderen M., Tharaux P.L., Melino G., Pacaud P. and Loirand G. Transglutaminase-dependent RhoA activation and depletion by serotonin in vascular smooth muscle cells. J. Biol. Chem. 2007;282(5):2918-28. DOI: 10.1074/jbc.M604195200
  • Loirand G., Guilluy C. and Pacaud P. Regulation of Rho proteins by phosphorylation in      the cadiovascular system. Trends Cardiovasc Med. 2006 Aug;16(6):199-204. DOI: 10.1016/j.tcm.2006.03.010
  • Gouëffic Y., Guilluy C., Guérin P., Patra P., Pacaud P., Loirand G. Hyaluronan induces vascular smooth muscle cell migration through RHAMM-mediated PI3K-dependent Rac activation. Cardiovasc Res. 2006 Nov 1;72(2):339-48. Epub      2006 Jul 29. DOI: 10.1016/j.cardiores.2006.07.017
  • Guilluy C., Sauzeau V.,      Rolli-Derkinderen M., Guérin P., Sagan C., Pacaud P. and Loirand G.      Inhibition of RhoA/Rho kinase pathway is involved in the beneficial effect      of sildenafil on pulmonary hypertension. Br. J. Pharmacol.     2005 Dec;146(7). DOI: 10.1038/sj.bjp.0706408


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